Hispanic Subgroups, Acculturation, and Substance Abuse Treatment Outcomes

This study explored Hispanic subgroup differences in substance use treatment outcomes, and the relationship of acculturation characteristics to these outcomes. Data were from a multisite randomized clinical trial of motivational enhancement therapy versus treatment as usual in a sample of Spanish-speaking substance abusers. Participants were Cuban American (n = 34), Mexican American (n = 209), Puerto Rican (n = 78), and other Hispanic American (n = 54). Results suggested that Cuban Americans and individuals with more connection to Hispanic culture had lower treatment retention. Hispanics born in the U.S and those who spoke English at home had a lower percentage of days abstinent during weeks 5–16, although Puerto Ricans born in the U.S. and Cuban Americans living more years in the U.S. had a higher percentage of days abstinent in weeks 1–4 and 5–16, respectively. Results may inform future hypothesis-driven studies in larger Hispanic treatment seeking samples of the relationship between acculturation and treatment outcome

From Structural Inequalities to Speaking Out: Youth Participatory Action Research in College Access Collaborations

In recent years, participatory action research projects aimed at addressing local social issues have gained interest in academic settings. These projects can contribute to university-community partnerships, but communication about such projects remains somewhat limited. This article contributes to these developing discussions by describing how a youth participatory action research project (YPAR) supported an ongoing university-community partnership between South University, a mid-sized private liberal arts school, and the local public school community. This educational partnership led to the Anchor Academy, a college access and success program for high school youth with limited financial resources and little or no family history of college. In 2010-2011, Anchor initiated a YPAR project to study the challenges limited-income, first-generation, and minority students faced on their path to college. This article describes how the project deepened university-community relationships, shaped broader awareness and local programming, and inspired a ripple effect of new partnerships that help to sustain the work of supporting marginalized students in their journeys toward college futures. It addresses the struggles faced by the project as well as the positive outcomes, ultimately arguing for the potential for critical, participatory research methodologies to serve as a particularly meaningful platform for collaboration between universities and their communities

Epigenetic reprogramming and emerging epigenetic therapies in CML

Chronic myeloid leukemia (CML) is a hematopoietic stem cell disorder characterized by BCR-ABL1, an oncogenic fusion gene arising from the Philadelphia chromosome. The development of tyrosine kinase inhibitors (TKIs) to overcome the constitutive tyrosine kinase activity of the BCR-ABL protein has dramatically improved disease management and patient outcomes over the past 20 years. However, the majority of patients are not cured and developing novel therapeutic strategies that target epigenetic processes are a promising avenue to improve cure rates. A number of epigenetic mechanisms are altered or reprogrammed during the development and progression of CML, resulting in alterations in histone modifications, DNA methylation and dysregulation of the transcriptional machinery. In this review these epigenetic alterations are examined and the potential of epigenetic therapies are discussed as a means of eradicating residual disease and offering a potential cure for CML in combination with current therapies

Antibody-based detection of protein phosphorylation status to track the efficacy of novel therapies using nanogram protein quantities from stem cells and cell lines

This protocol describes a highly reproducible antibody-based method that provides protein level and phosphorylation status information from nanogram quantities of protein cell lysate. Nanocapillary isoelectric focusing (cIEF) combines with UV-activated linking chemistry to detect changes in phosphorylation status. As an example application, we describe how to detect changes in response to tyrosine kinase inhibitors (TKIs) in the phosphorylation status of the adaptor protein ​CrkL, a major substrate of the oncogenic tyrosine kinase ​BCR-​ABL in chronic myeloid leukemia (CML), using highly enriched CML stem cells and mature cell populations in vitro. This protocol provides a 2.5 pg/nl limit of protein detection (<0.2% of a stem cell sample containing <104 cells). Additional assays are described for phosphorylated tyrosine 207 (pTyr207)-​CrkL and the protein tyrosine phosphatase ​PTPRC/​CD45; these assays were developed using this protocol and applied to CML patient samples. This method is of high throughput, and it can act as a screen for in vitro cancer stem cell response to drugs and novel agents

Autoimmune hyperphosphatemic tumoral calcinosis in a patient with FGF23 autoantibodies

Hyperphosphatemic familial tumoral calcinosis (HFTC)/hyperostosis-hyperphosphatemia syndrome (HHS) is an autosomal recessive disorder of ectopic calcification due to deficiency of or resistance to intact fibroblast growth factor 23 (iFGF23). Inactivating mutations in FGF23, N-acetylgalactosaminyltransferase 3 (GALNT3), or KLOTHO (KL) have been reported as causing HFTC/HHS. We present what we believe is the first identified case of autoimmune hyperphosphatemic tumoral calcinosis in an 8-year-old boy. In addition to the classical clinical and biochemical features of hyperphosphatemic tumoral calcinosis, the patient exhibited markedly elevated intact and C-terminal FGF23 levels, suggestive of FGF23 resistance. However, no mutations in FGF23, KL, or FGF receptor 1 (FGFR1) were identified. He subsequently developed type 1 diabetes mellitus, which raised the possibility of an autoimmune cause for hyperphosphatemic tumoral calcinosis. Luciferase immunoprecipitation systems revealed markedly elevated FGF23 autoantibodies without detectable FGFR1 or Klotho autoantibodies. Using an in vitro FGF23 functional assay, we found that the FGF23 autoantibodies in the patient's plasma blocked downstream signaling via the MAPK/ERK signaling pathway in a dose-dependent manner. Thus, this report describes the first case, to our knowledge, of autoimmune hyperphosphatemic tumoral calcinosis with pathogenic autoantibodies targeting FGF23. Identification of this pathophysiology extends the etiologic spectrum of hyperphosphatemic tumoral calcinosis and suggests that immunomodulatory therapy may be an effective treatment

NASA Light Emitting Diode Medical Applications from Deep Space to Deep Sea

This work is supported and managed through the NASA Marshall Space Flight Center-SBIR Program. LED-technology developed for NASA plant growth experiments in space shows promise for delivering light deep into tissues of the body to promote wound healing and human tissue growth. We present the results of LED-treatment of cells grown in culture and the effects of LEDs on patients’ chronic and acute wounds. LED-technology is also biologically optimal for photodynamic therapy of cancer and we discuss our successes using LEDs in conjunction with light-activated chemotherapeutic drugs

Use of a onedimensional link-node model to develop total maximum daily load strategies for the San Joaquin River Estuary

A one-dimensional link-node model was used to simulate water quality conditions in the tidallyinfluenced, deep water ship channel (DWSC) of the San Joaquin River located in Central California. The DWSC has been plagued with low dissolved oxygen (DO) conditions for decades and is currently a focus of restoration efforts. The model was calibrated using a six-year flow and water quality data set. Model simulations were run by removing the mass loads of each of the following major sources of oxygen depletion to determine the effects: elimination of the deepened ship channel (i.e., restore to its preexisting depth), elimination of import of oxygen-demanding substances (ODS) from the San Joaquin River watershed, elimination of import of ODS from the urban tributaries, and elimination of discharge of ODS from the City of Stockton regional wastewater control facility. The model results suggest that elimination of the deepened ship channel resulted in the best projected improvement relative to the modelled baseline with a predicted 55% improvement, while reducing ODS from the watershed would likely cause a 44% improvement. These results demonstrate that there are multiple contributing factors causing low DO in the DWSC and that removal or elimination of any single variable will not result in a complete resolution of low DO events

Use of the WARMF Model to Identify Sources of Oxygen Impairment and Potential Management Strategies for the San Joaquin River Watershed

Eutrophication of the San Joaquin River (SJR) has resulted in low dissolved oxygen (DO) conditions, which has led to a regulatory response and development of total maximum daily load (TMDL) allocations. Due to the dynamic nature of processes governing oxygen depletion in the SJR, a model was needed to help stakeholders understand the fate and transport of nutrients and oxygendemanding substances that cause the low DO conditions. Here, the Watershed Analysis Risk Management Framework (WARMF) model was used to simulate nutrient removal and control strategies, accounting for the secondary effects of growth and transformation between sources and discharge. Using the management alternatives in the WARMF Consensus Module, simulations were run to test the global removal of nutrient inputs on downstream phytoplankton growth, a major contributor of oxygen demand in the SJR. In the simulations, removal of ammonia had the greatest impact on downstream phytoplankton, causing a 32% reduction, while removal of phosphate and nitrate caused reductions of 25% and 13%, respectively. When ammonia and nitrate were both removed, phytoplankton reduction was 62%. These model results suggest that nitrogen control programs would be more effective than phosphorus programs. Using the Data Module in WARMF, input files were modified to determine the impacts of individual tributaries and agricultural drainages. In each simulation, the contributing loads for individual inputs were removed while maintaining flow. According to the model output, the largest impact on phytoplankton occurred with the removal of mass loads from Salt Slough (32% less than baseline). The effect of removing the mass loads from Mud Slough had a slightly lower impact (26% less than baseline). The WARMF model proved useful for exploration of planning and management alternatives, providing an expert decision-making tool that is available to stakeholders

Exploring the Requisite Skills and Competencies of Pharmacists Needed for Success in an Evolving Health Care Environment

Objective. To identify and describe the core competencies and skills considered essential for success of pharmacists in today’s rapidly evolving health care environment

Graphics mini manual

The computer graphics capabilities available at the Center are introduced and their use is explained. More specifically, the manual identifies and describes the various graphics software and hardware components, details the interfaces between these components, and provides information concerning the use of these components at LaRC